Medical-grade extracellular vesicles derived from umbilical wire mesenchymal stromal cells: preclinical growth and first-in-human intra-articular validation as therapeutics for knee osteoarthritis | Journal of Nanobiotechnology
Non-clinical (non cGMP) sEV manufacturing and enrichment Preclinical sEV batches had been obtained as reported beforehand [20]. Briefly, UC-MSC from three donors in passage 5 had been seeded and expanded in a upkeep medium composed of Dulbecco’s modified Eagle’s medium (DMEM), excessive glucose, supplemented with 1% penicillin/streptomycin resolution (10,000 U/mL and 10,000 μg/mL, respectively), 1% L-glutamine (200 mM)…