Nebulized milk exosomes loaded with siTGF-β1 ameliorate pulmonary fibrosis by inhibiting EMT pathway and enhancing collagen permeability | Journal of Nanobiotechnology
Isolation and characterization of M-Exos Exosomes have been remoted from uncooked milk by differential centrifugation strategies in accordance with beforehand established protocols [39, 40]. Briefly, contemporary milk underwent centrifugation at 5,000 × g for 20 min at 4 °C, adopted by a second centrifugation at 12,000 × g for 60 min at 4 °C to eradicate the remaining fats…